• Next-Generation Sequencing (NGS)

    SOURCE: From a leading company in Genomics, Illumina

    VIDEOS

    Intro to NGS
    NGS Animation
    Illumina’s NSG Method
    Illumina’s Sequencing Tech
    Ion Torrent’s Sequencing
    Roche’s NGS

    (See a more in-depth study of NGS, in this Illumina PDF)

    From Wikepedia, …”Nextgeneration sequencing (NGS), also known as high-throughput sequencing, is the catch-all term used to describe a number of different modern sequencing technologies including: Illumina (Solexa) sequencing. Roche 454 sequencing. Ion torrent: Proton / PGM sequencing. SOLiD sequencing.

    Here follows, Illumina’s take on NSG:

    “With its unprecedented throughput, scalability, and speed, next-generation sequencing enables researchers to study biological systems at a level never before possible.

    Today’s complex genomic research questions demand a depth of information beyond the capacity of traditional DNA sequencing technologies. Next-generation sequencing has filled that gap and become an everyday research tool to address these questions.

    See What NGS Can Do For You

    Innovative NGS sample preparation and data analysis options enable a broad range of applications. Next-gen sequencing allows you to:

    Accessible Whole-Genome Sequencing

    Using capillary electrophoresis-based Sanger sequencing, the Human Genome Project took over 10 years and cost nearly $3 billion.

    Next-generation sequencing, in contrast, makes large-scale whole-genome sequencing accessible and practical for the average researcher.

    Limitless Dynamic Range for Expression Profiling

    NGS makes sequence-based gene expression analysis a “digital” alternative to analog techniques. It lets you quantify RNA expression with the breadth of a microarray and the resolution of qPCR.

    Microarray gene expression measurement is limited by noise at the low end and signal saturation at the high end. In contrast, next-generation sequencing quantifies discrete, digital sequencing read counts, offering a virtually unlimited dynamic range.

    Tunable Resolution for Targeted Next-Gen Sequencing

    NGS is highly scalable, allowing you to tune the level of resolution to meet specific experimental needs.

    Targeted sequencing allows you to focus your research on particular regions of the genome. Choose whether to do a shallow scan across multiple samples, or sequence at greater depth with fewer samples to find rare variants in a given region.

    How Does Illumina NGS Work?

    Illumina next-generation sequencing utilizes a fundamentally different approach from the classic Sanger chain-termination method. It leverages sequencing by synthesis (SBS) technology – tracking the addition of labeled nucleotides as the DNA chain is copied – in a massively parallel fashion.

    Next-gen sequencing generates masses of DNA sequence data that’s richer and more complete than is imaginable with Sanger sequencing. Illumina sequencing systems can deliver data output ranging from 300 kilobases up to 1 terabase in a single run, depending on instrument type and configuration.

    Learn more about sequencing by synthesis (SBS) technology »

    Latest Evolution of Illumina Next-Gen Sequencing

    Recent Illumina next-generation sequencing technology breakthroughs include:

    Bring Next-Generation Sequencing to Your Lab

    The following resources offer valuable guidance to researchers who are considering purchasing an NGS system:

     

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