• .Confocal microscope image of liposome nanocapsules containing bacteriophages


    June 12, 2016

    (Nanowerk News) Scientists at the Universitat Autònoma de Barcelona (UAB) and the Catalan Institute for Nanoscience and Nanotechnology (ICN2) have developed a nanoencapsulation system with a liposome coating in order to increase the efficacy of bacteriophages in oral phage therapy (Applied and Environmental Microbiology, “Liposome-Encapsulated Bacteriophages for Enhanced Oral Phage Therapy Against Salmonella”).The research demonstrated that a liposome nanoencapsulation provides the bacteriophage with greater resistance to stomach acids and increases residence time in the intestinal tract of model broiler chickens in simulated poultry farming conditions. The technology developed could be applied to bacteriophages with different morphologies to be used in phage therapy, in both animals and humans

    Oral phage therapy has demonstrated to be a feasible and effective tool in the control of infections caused by different bacterial pathogens. In previous studies, the UAB Molecular Microbiology Group had published the isolation and characterisation of three virulent bacteriophages (UAB_Phi20, UAB_Phi78, and UAB_Phi87) specific to Salmonella, and demonstrated their efficacy in the reduction of the concentration of this zoonotic bacteria in models of specific pathogen-free (SPF) White Leghorn chickens, and in several experimentally contaminated food matrices. Nevertheless, in this research two limitations were observed in the use of orally administered bacteriophages: the reduced stability of the phages in extremely acid environments, such as the stomach, and short residence time in the intestinal tract.

    To overcome these limitations, researchers developed a nanoencapsulation system using liposome capsules and applied them to the three aforementioned virulent bacteriophages in order to compare the effects of liposome-encapsulated phages and nonencapsulated phages on the concentration of Salmonella in model broiler chickens experimentally contaminated with the bacteria. The experiment was conducted at the UAB Farms and Experimental Fields Services, with all the conditions of a real poultry farm.Thanks to the study, nanometric capsules were developed, with an average diameter of 320 nm and a positive charge of 33mV.

    The nanocapsules containing the bacteriophages were observed using a cryo-electron microscope (Cryo-TEM) and confocal microscope. Researchers observed how the liposome coating allowed the encapsulated bacteriophages to be significantly more stable in the gastric fluids. The coating also significantly improved the time the bacteriophages stayed inside the intestinal tract of the chickens. After 72 hours encapsulated bacteriophages were detected in 38.1% of animals, while only 9.5% of animals showed signs of still containing the nonencapsulated bacteriophages.In oral therapy experiments, once the treatment was suspended, the protection provided by nonencapsulated bacteriophages disappeared, while the encapsulated ones were effective for at least another week.

    The methodology developed allows encapsulating bacteriophages of different sizes and morphologies, demonstrates the advantages of using encapsulated bacteriophages for oral phage therapy and, moreover, the nanometric size allows adding it to potable water and fodder.The research was jointly conducted by the UAB Molecular Microbiology Group from the Department of Genetics and Microbiology, directed by Montserrat Llagostera, and the Supramolecular NanoChemistry & Materials group at the ICN2, directed by ICREA professor Daniel Maspoch. The work was published recently in the journal Applied and Environmental Microbiology and is part of the PhD thesis with international mention by Joan Colom Comas entitled “Studies of the Molecular features of Three Salmonella Phages for Use in Phage Therapy and of Encapsulation Methodologies to Improve Oral Phage Administration”.

    Source: Universitat Autònoma de Barcelona

    Read more: Virus nanocapsules to treat infections


    No comments

    Be the first one to leave a comment.

    Post a Comment



    Latest Posts

    Latest Video



    First FDA Approval For Clinical Cloud-Based Deep Learning In Healthcare

     SOURCE: FORBES The first FDA approval for a machine learning application to be used in a clinical setting is a…

    FDA-approved study uses adipose stem cells for treatment of shoulder injuries

    SOURCE Sanford Health is conducting the first clinical trial approved by the FDA to treat injured shoulders using patients’ adipose…

    Could Anatomics BioModel Stereotaxy replace navigation or a robot?

    SOURCE Paul S D’Urso Neurosurgeon & Founder of Anatomics Because 3D printing is so accurate I was able to locate…

    $250 million awarded to new Advanced Robotics Manufacturing Innovation Hub

    SOURCE Headquartered in Pittsburgh, PA, the new institute is made up of governments, industry, academia, and non-profit organizations from across the country….

    Self-assembling 3D printing inks produce more true-to-life tissue

    SOURCE Biological and chemical researchers are developing 3D printable inks capable of self-assembly, i.e. forming a shape based on a…

    Urine Exosomes – An Emerging Trove of Biomarkers

     SOURCE Exosomes are released by most cells and can be isolated from all biofluids including urine. Exosomes are small vesicles…

    What is machine learning?

    SOURCE In this fascinating animation from Oxford Sparks, we take a look at how statistics and computer science can be used to…

    Catalia Health uses social robots to improve health outcomes

    Credit: Catalia Health SOURCE Catalia Health is leading the surge in social robotics, with Mabu, their patient care management system. Catalia…