ESO-BRAIN and light sheet imaging techniques
MESO-BRAIN initiative receives €3.3million to replicate brain’s neural networks through 3D nanoprinting.The MESO-BRAIN consortium has received a prestigious award of €3.3million in funding from the European Commission as part of its Future and Emerging Technology (FET) scheme. The project aims to develop three-dimensional (3D) human neural networks with specific biological architecture, and the inherent ability to interrogate the network’s brain-like activity both electrophysiologically and optically. It is expected that the MESO-BRAIN will facilitate a better understanding of human disease progression, neuronal growth and enable the development of large-scale human cell-based assays to test the modulatory effects of pharmacological and toxicological compounds on neural network activity. The use of more physiologically relevant human models will increase drug screening efficiency and reduce the need for animal testing.
The MESO-BRAIN project’s cornerstone will use human induced pluripotent stem cells (iPSCs) that have been differentiated into neurons upon a defined and reproducible 3D scaffold to support the development of human neural networks that emulate brain activity. The structure will be based on a brain cortical module and will be unique in that it will be designed and produced using nanoscale 3D-laser-printed structures incorporating nano-electrodes to enable downstream electrophysiological analysis of neural network function. Optical analysis will be conducted using cutting-edge light sheet-based, fast volumetric imaging technology to enable cellular resolution throughout the 3D network. The MESO-BRAIN project will allow for a comprehensive and detailed investigation of neural network development in health and disease.
Prof Edik Rafailov, Head of the MESO-BRAIN project (Aston University) said: “What we’re proposing to achieve with this project has, until recently, been the stuff of science fiction. Being able to extract and replicate neural networks from the brain through 3D nanoprinting promises to change this. The MESO-BRAIN project has the potential to revolutionise the way we are able to understand the onset and development of disease and discover treatments for those with dementia or brain injuries. We cannot wait to get started!”
The MESO-BRAIN project will launch in September 2016 and research will be conducted over three years.
ICFO- The Institute of Photonic Sciences is one of the members of this project, providing the know-how and expertise on light sheet imaging techniques as a tool to observe and study the growth of realistic and reproducible functional neuronal networks. Dr. Pablo Loza, head of the SLN facility at ICFO, explains, “It is a great opportunity to be able to participate in such an extraordinary project in which realistic 3D neuron communication will be fully studied. Understanding how such communication patterns occur would have enormous implications, allowing us to move a step forward in fighting neuronal degeneration diseases and brain injuries, conditions that, for the moment, cannot be cured.