The Attune® Acoustic Focusing Cytometer was used to evaluate genetic marker expression in the iPSC and NSC populations.
Disease Modeling Using Stem Cells
September 15, 2014
Stem cells show promise in many research areas, especially in disease modeling. By reprogramming donated somatic cells exhibiting disease morphology, a potentially unlimited source of induced pluripotent stem cells (iPSCs) offers the ability to expand, differentiate, and study affected human cells without another animal model. Physiologically relevant cellular models can accelerate the discovery of disease mechanisms. We can help you develop human disease models using our tools and technologies, and manage the technical challenges that arise.
How it was done: building the disease cell models
We have collaborated with the Parkinson’s Institute in Sunnyvale, California, to demonstrate how to develop cellular models to identify drugs that may ameliorate the underlying processes of Parkinson’s disease (PD).
In a series of white papers, we describe our case studies for creating PD cell models using Life Technologies tools.
Research protocols & resources
We created, modified, and used many protocols while building the cellular model case studies. Listed here are the most popular protocols and media used in the stem cell workflow typically employed in our research, grouped into culture, engineering, differentiation, and characterization workflow segments. Go to Disease Modeling Resources to find a more comprehensive set of protocols and resources, including webinars, scientific posters, and more.
Learn about the products used in the research for these case studies
We bring new Life Technologies tools to the market that accelerate scientific research, giving us access to an increasing number of researchers globally and enabling research in disease areas not previously possible. Read how we used these items in our collaboration and learn how you can apply them in your lab.
The Ion PGM™ Sequencer was used to evaluate the donor fibroblasts for additional mutations prior to reprogramming.
Essential 8™ Medium was the feeder-free growth medium we used for iPSCs once they were reprogrammed from the donor fibroblasts.
Gibco® PSC Neural Induction Medium (NIM) allowed us to generate NSCs from iPSCs within 7 days without the need for embyoid body (EB) formation.
The CytoTune®-iPS Sendai Reprogramming Kit was used to reprogram donor fibroblasts into iPSCs. The resulting iPSCs are integration-free and have no risk of potential tumorigenicity.
The EVOS® FLoid® Cell Imaging Station enabled the evaluation of cells for pluripotency and neural cell markers at various stages via fluorescence immunocytochemistry.
The QuantStudio™ 12K Flex Real-Time PCR System was used for gene expression analysis of the iPSCs, NSCs, and differentiated neural cells in various characterization experiments, and as an iPSC colony screening method through SNP genotyping analysis.
The TaqMan® hPSC Scorecard™ Panel is a PCR assay we used to evaluate pluripotency and confirm trilineage differentiation potential in the iPSCs.
CRISPR-Cas systems are revolutionizing the field of genome editing through the technologies’ ability to achieve highly flexible and specific targeting of desired genome edits. See how you can get started today.